The bacteria being utilized in this experimentation will be unknown gram negative bacterium number 22 that had been inoculated in a Tryptic Soy Broth. During the whole process the caps and the tubes were held in hand were not allowed to be contaminated by contacting the table or other source of environmental bacteria. Once a gram-stain procedure was completed, the gram-positive unknown was identified as rod-shaped. Antibiotic resistance, Bacteria, Escherichia coli 1090 Words 5 Pages The Periodic Law Lab Objectives: 1. One strand in particular, O157:H7, is a harmful strand that contaminates water and food that humans or animals could ingest 4. When I say the borders of the bacterial colonies are irregular, I mean that both the shape is irregular and the consistency of the borders is not like the center of the colony. How did the student come in contact with this organism? Casein test and a Matlose test were performed.
Then we determined the volume of the unknown solid by water displacement. Furthermore, the Unknown J did not produce or metabolize starch. Table 1: test performed to find out what gram negative bacteria is it, by following the procedures. The gram stain is a differential stain that utilizes a primary stain of crystal violet i. Phan Cong Hoang for helpful discussions during the preparation of this thesis report and for providing the bacterial strains. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This method was carried out by streaking a nutrient agar plate in four different directions, in hopes of isolating two different unknowns.
This experiment was done by applying methods in order to identify an unknown bacterium. The bacterial growth seems to be spreading more rapidly on this slant. In order to determine the morphology of the bacteria a gram-stain followed the T-streak. These two organisms have opposite test results in the Urea test. When the Bacillus subtilis was isolated on the Mannitol Salt Agar plate, the color of the plate also changed from red to yellow. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function.
After the second attempt at inoculation, two species were seen with the naked eye. Introduction All organisms are divided into three domains; bacteria, archaea, and eukarya. The lid was then put back on the plates and the loop was again sterilized by burning until glowing bright red on the Bunsen burner. The aim of this document is to provide a general guideline on writing a lab report. Biotechnologists can also exploit the activities of microbes to benefit humans, such as in the production of medicines, enzymes and food. Scientist use Bacillus subtilis in order to promote genetic research and due to its highly genetic manipulability levels, it is easier to conduct testing on Swartzburg, 2009.
Third, a maltose test was performed on the Gram positive bacterium. The purpose of this lab was to collect and observe microbes from environmental and human body samples by culturing them on the appropriate media using aseptic transfer techniques. When Gram positive bacteria are treated with lysozyme. The tube in 25oC: The amount of growth was slight, white in color, and had Echinulate form of growth. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. In gram staining, besides gram reaction, the morphology and arrangement of the bacteria are also recorded.
The experiment was done by applying methods in order to identify an unknown bacterium. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology. After the streak for isolation is completed, a gram stain will be done. Words: 1847 - Pages: 8. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. All healthcare providers ultimately have to take a microbiology class as part of their program curriculum. Oxidative metabolic tests for oxidase and catalase were also performed using an oxidative reagent and hydrogen peroxide, respectively.
Specifically, this gas acts as a nucleus for cloud formation which reduces the amount of radiation from the sun that. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. The citrate test truly helped confirm that the unknown bacteria 25 was in fact Escherichia coli. Bacteria prepared for the Gram stain is a heated fixed smear that is covered with a crystal violet. There is a specific style that must be followed when writing scientific reports. Then it was confirmed by the instructor that it was the right gram negative found.
The experiment was done by applying methods in order to identify an unknown bacterium. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. Materials and Methods Over a two week period, eight prepared types of test media were provided to identify the assigned unknown mixed. A flame sterilized inoculating loop with bacterial growth was stirred around the medium. The two easiest ways that humans can be infected are by ingesting contaminated water or ingesting contaminated food. Please note that due to formatting issues the flow charts had to be removed.
The lid of the plates was opened with free hand and the material was seeded with gentle strokes of the loop using T streak method in order to allow optimal contidions for growth. When it was determined that it was a gram-negative rod due to its tube-shaped features and red staining, further testing needed to be done to eliminate potential organisms. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. These tests were both negative, which confirmed that culture 2 was Bacillus subtilis. Some other ways include, having physical contact, contact with animals and high fiber diets 2. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class.
The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. This serum, containing antibody against that bacteria is called the antiserum of that bacteria. Therefore, the first necessary step was to streak plate and inoculate the unknown before another gram-staining procedure could be completed. Materials and Methods A test tube with an unknown microorganism will be retrieved. The pattern of growth is bitted. The Voges-Proskauer test came back with a negative result.